GM03234
Fibroblast from Skin, Arm
Description:
APPARENTLY HEALTHY INDIVIDUAL
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Apparently Healthy Collection |
Biopsy Source
|
Arm
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Arm
|
Race
|
American Indian/Alaska Native
|
Ethnicity
|
Pima Indian
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis and Case history
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
PDL at Freeze |
5.68 |
Passage Frozen |
6 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
PubMed ID: 30567591 |
|
Goldman D, Giri PR, O'Brien SJ, A molecular phylogeny of the hominoid primates as indicated by two- dimensional protein electrophoresis. Proc Natl Acad Sci U S A84:3307-11 1987 |
PubMed ID: 3106965 |
|
Goldman D, Goldin LR, Rathnagiri P, O'Brien SJ, Egeland JA, Merril CR, Twenty-seven protein polymorphisms by two-dimensional electrophoresis of serum, erythrocytes, and fibroblasts in two pedigrees. Am J Hum Genet37:898-911 1985 |
PubMed ID: 3863481 |
|
Howard BV, Fields RM, Mott DM, Savage PJ, Nagulesparan M, Bennett PH, Diabetes and cell growth--lack of differences in growth characteristics of fibroblasts from diabetic and nondiabetic Pima Indians. Diabetes29:119-24 1980 |
PubMed ID: 7353728 |
Passage Frozen |
6 |
Split Ratio |
1:2 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Supplement |
- |
|
|