NG09266
DNA from Fibroblast
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
Repository
|
NIA Aging Cell Culture Repository
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Subcollection |
GRC |
Quantity |
10ug |
Quantitation Method |
Please see our FAQ |
Biopsy Source
|
Arm
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
DNA from Fibroblast
|
Race
|
White
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Freeze |
5.64 |
Passage Frozen |
2 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY/46,XY,t(1;17)(q21;q21)/46,XY,t(1;17)(q21;q21),t(11; 16)(q13;q13); 84%/2%/12%; balanced with 2% of the cells showing random chromosome loss. Donor is the son of AG05844A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
Xiong ZM, Mao X, Trappio M, Arya C, Kordi JE, Cao K, Ultraviolet radiation protection potentials of Methylene Blue for human skin and coral reef health Scientific reports11:10871 2021 |
PubMed ID: 34050204 |
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Reed, TGF-beta 1 induces the expression of type 1 collagen and SPARC, and enhances contraction of collagen gels, by fibroblasts from young and aged donors. J Cell Biol158:169 (1994):10871 1994 |
PubMed ID: 34050204 |
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