AG08260
Fibroblast from Skin, Arm
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Alzheimer's Disease |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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4
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Relation to Proband
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paternal uncle
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
4.64 |
| Passage Frozen |
6 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
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| Remarks |
Donor is a probable escapee in an Alzheimer's disease family. Donor's deceased brother was affected. The skin biopsy was taken ante-mortem from the forearm. Culture was initiated on 6/19/85 using explants of minced skin. The cell morphology is fibroblast-like. Culture was frozen at PDL 4. Donor's affected niece is AG08744 Lymphblast. A lymphoblast culture from same donor is AG08257. |
| Cumulative PDL at Freeze |
8.64 |
| Passage Frozen |
6 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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