AG22359
Fibroblast from Lung, Lung
Description:
PEROMYSCUS
ORDER: RODENTIA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Family Member
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1
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Family History
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N
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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49,XX,+1,inv(16)(pter>H::A3>H::A3>qter)[7]/48,XX,inv(16)(pter>H::A3>H::A3>qter)[43]
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Species
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Peromyscus peromyscus maniculatus
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Common Name
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deer mouse
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Remarks
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| PDL at Senescence |
16 |
| PDL at Freeze |
2.72 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Peromyscus peromyscus maniculatus (deer mouse); lung fibroblast culture established on 4/24/2008 from explants of minced lung tissue |
| PDL at Senescence |
16 |
| Cumulative PDL at Freeze |
2.72 |
| Passage Frozen |
2 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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