AG21329
Fibroblast from Skin, Ear
Description:
MACACA FASCICULARIS
ORDER: PRIMATES
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Nonhuman Primates Animals |
Biopsy Source
|
Ear
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Ear
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
Species
|
Macaca fascicularis
|
Common Name
|
crab-eating macaque
|
Remarks
|
|
PDL at Freeze |
6.72 |
Passage Frozen |
4 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Remarks |
Macaca fascicularis from Indonesia with an estimated age of 10 years; skin fibroblast cell culture from explants of ear tissue |
Cumulative PDL at Freeze |
9.11 |
Passage Frozen |
4 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Supplement |
- |
|
|