AG16605
Endothelial from Aorta, abdominal
Description:
PAPIO ANUBIS
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Aorta, abdominal
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, abdominal
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Ethnicity
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TANZANIAN
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Family Member
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1
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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ISCN
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42,XX
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Species
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Papio anubis
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Common Name
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olive baboon
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Remarks
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| PDL at Senescence |
59 |
| PDL at Freeze |
8 |
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
The abdominal aorta biopsy was taken ante-mortem on 12/19/00. |
| PDL at Senescence |
59 |
| Cumulative PDL at Freeze |
8 |
| Passage Frozen |
2 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 medium/Medium 199, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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