AG15897
Fibroblast from Skin, Unspecified
Description:
MUS
ORDER: RODENTIA
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Unspecified
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Unspecified
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
63-64,XXXX,-2,+3,+5,-7,+10,+11,-13,-14,-16,+mar1,+mar2,+mar3,+mar4 cpk[9]
|
|
Species
|
Mus musculus
|
|
Common Name
|
house mouse
|
|
Remarks
|
|
| PDL at Freeze |
4 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Genbank |
COX1 |
| Remarks |
Spontaneously transformed mouse fibroblast culture. The line is hypertriploid and has a karyotype of 63-64,XXXX,-2,+3,+5,-7,+10,+11,-13,-14,-16,+mar1,+mar2,+mar3,+mar4 cpk[9]. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Cumulative PDL at Freeze |
4 |
| Split Ratio |
1:9 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
5% fetal bovine serum Heat Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Sodium Pyruvate 100mM |
|
|