AG12256
Fibroblast from Skin, Skin
Description:
PONGO PYGMAEUS
ORDER: PRIMATES
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Nonhuman Primates
Animals |
|
Biopsy Source
|
Skin
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Skin
|
|
Ethnicity
|
SUMATRAN
|
|
Family Member
|
2
|
|
Relation to Proband
|
daughter
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
48,XX
|
|
Species
|
Pongo pygmaeus
|
|
Common Name
|
Sumatran orangutan
|
|
Remarks
|
|
| Remarks |
Sumatran orangutan. Culture was passaged twice at Coriell and the PDL of the freeze is unknown. Donor is daughter of AG12255. |
| Babcock M, Yatsenko S, Stankiewicz P, Lupski JR, Morrow BE, AT-rich repeats associated with chromosome 22q112 rearrangement disorders shape human genome architecture on Yq12 Genome research17:451-60 2007 |
| PubMed ID: 17284672 |
| |
| Fortna A, Kim Y, MacLaren E, Marshall K, Hahn G, Meltesen L, Brenton M, Hink R, Burgers S, Hernandez-Boussard T, Karimpour-Fard A, Glueck D, McGavran L, Berry R, Pollack J, Sikela JM, Lineage-specific gene duplication and loss in human and great ape evolution. PLoS Biol2(7):E207 2004 |
| PubMed ID: 15252450 |
| |
| Otieno AC, Carter AB, Hedges DJ, Walker JA, Ray DA, Garber RK, Anders BA, Stoilova N, Laborde ME, Fowlkes JD, Huang CH, Perodeau B, Batzer MA, Analysis of the human Alu Ya-lineage. J Mol Biol342(1):109-18 2004 |
| PubMed ID: 15313610 |
| |
| Janczewski DN, Goldman D, O'Brien SJ, Molecular genetic divergence of orang utan (Pongo pygmaeus) subspecies based on isozyme and two-dimensional gel electrophoresis. J Hered81:375-87 1990 |
| PubMed ID: 2230076 |
| PDL at Senescence |
51 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|