AG07184
Smooth muscle from Aorta, thoracic
Description:
CRICETULUS
ORDER: RODENTIA
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Aorta, thoracic
|
|
Cell Type
|
Smooth muscle
|
|
Tissue Type
|
Artery
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Smooth muscle from Aorta, thoracic
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Mesocricetus auratus
|
|
Common Name
|
golden (or syrian) hamster
|
|
Remarks
|
|
| PDL at Freeze |
12 |
| Passage Frozen |
5 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Golden Syrian hamster strain LAK:LVG SYR (Charles River outbred Lakeland line). The aorta was taken immediately post-mortem on 11/29/83. Strips of the medial layer were removed, minced, and used as explants to initiate the culture. Cell morphology is fibroblast-like and the cells form layered sheets at post-confluence. The culture tested positive for alpha-smooth muscle actin (immunofluorescence assay). Culture grows slowly. |
| Cumulative PDL at Freeze |
12 |
| Passage Frozen |
5 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|