AG07100
Fibroblast from Skin, Arm
Description:
LEMUR CATTA
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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56,XX,inv(10q)
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Species
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Lemur catta
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Common Name
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ring-tailed lemur
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Remarks
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| PDL at Senescence |
31 |
| PDL at Freeze |
13 |
| Passage Frozen |
7 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electro-{ phoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
The skin biopsy was obtained 10/17/83, ante-mortem, from the inner aspect of the upper left arm. |
| Diehl WE, Stansell E, Kaiser SM, Emerman M, Hunter E, Identification of Post-entry Restrictions to Mason-Pfizer Monkey Virus Infection in New World Monkey Cells Journal of virology82(22):11140-50 2008 |
| PubMed ID: 18799582 |
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| Horvath JE, Weisrock DW, Embry SL, Fiorentino I, Balhoff JP, Kappeler P, Wray GA, Willard HF, Yoder AD, Development and application of a phylogenomic toolkit: Resolving the evolutionary history of Madagascar's lemurs Genome research18:489-499 2008 |
| PubMed ID: 18245770 |
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| Steinert S, White DM, Zou Y, Shay JW, Wright WE, Telomere biology and cellular aging in nonhuman primate cells. Exp Cell Res272(2):146-52 2002 |
| PubMed ID: 11777339 |
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| Kenyon L,Moraes C, Expanding the functional human mitochondrial DNA database by the establishment of primate xenomitochondrial cybrids. Proc Natl Acad Sci U S A94:9131-5 1997 |
| PubMed ID: 9256447 |
| PDL at Senescence |
31 |
| Cumulative PDL at Freeze |
13 |
| Passage Frozen |
7 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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