AG06249
Fibroblast from Skin, Arm
Description:
MACACA MULATTA
ORDER: PRIMATES
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Nonhuman Primates Animals |
Biopsy Source
|
Arm
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Arm
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
42,XX[48]/41,XX-17[2]
|
Species
|
Macaca mulatta
|
Common Name
|
rhesus monkey
|
Remarks
|
|
PDL at Freeze |
8 |
Passage Frozen |
5 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
Genbank |
COX1 |
Remarks |
Indian strain of rhesus macaque; the skin biopsy was obtained 1/11/83, ante-mortem, from the inner aspect of the upper arm. |
Cumulative PDL at Freeze |
8 |
Passage Frozen |
5 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|