AG06105
Fibroblast from Skin, Thoraco-lumbar junction
Description:
PONGO PYGMAEUS
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Thoraco-lumbar junction
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Thoraco-lumbar junction
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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48,XX
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Species
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Pongo pygmaeus
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Common Name
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Sumatran orangutan
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Remarks
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| PDL at Freeze |
10 |
| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
The donor was captured in the wild at an estimated age of 6 years and thereafter reared in a zoo. Skin biopsy was taken ante-mortem on 10/12/82. Homozygous for Sumatran chromosome #2 polymorphism. |
| Ovchinnikov I, Rubin A, Swergold GD, Tracing the LINEs of human evolution. Proc Natl Acad Sci U S A99(16):10522-7 2002 |
| PubMed ID: 12138175 |
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| Schempp W, Weber B, Muller G, Mammalian sex-chromosome evolution: a conserved homoeologous segment on the X and Y chromosomes in primates. Cytogenet Cell Genet50:201-5 1989 |
| PubMed ID: 2805817 |
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| Weber B, Weissenbach J, Schempp W, Conservation of human-derived pseudoautosomal sequences on the sex chromosomes of the great apes. Cytogenet Cell Genet45(1):26-9 1987 |
| PubMed ID: 3474106 |
| Cumulative PDL at Freeze |
10 |
| Passage Frozen |
5 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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