AG05313
Fibroblast from Skin, Thigh
Description:
SAGUINUS FUSCICOLLIS
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Thigh
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Saguinus fuscicollis
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Common Name
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white-lipped or brown-headed tamarin
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Remarks
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| PDL at Freeze |
16 |
| Passage Frozen |
15 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Rheinemann L, Downhour DM, Davenport KA, McKeown AN, Sundquist WI, Elde NC, Recurrent evolution of an inhibitor of ESCRT-dependent virus budding and LINE-1 retrotransposition in primates Current biology : CB32:1511-1522.e6 2021 |
| PubMed ID: 35245459 |
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| Choby JE, Buechi HB, Farrand AJ, Skaar EP, Barber MF, Molecular Basis for the Evolution of Species-Specific Hemoglobin Capture by Staphylococcus aureus mBio9:1511-1522.e6 2018 |
| PubMed ID: 30459189 |
| Cumulative PDL at Freeze |
16 |
| Passage Frozen |
15 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides/Dulbecco Modified Eagles Medium (high glucose), 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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