AG04660
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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3
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Relation to Proband
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cousin
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,t(5;12)(5qter>5p15.3::12q13>12qter;12pter>12q13::5p15.3>5pter)[1]/45,X[2]/46,XY,t(1;5;18)(1pter>1q25::5q11.2>5qter;5pter>5q11.2::18q11.2>18qter;18pter>18q11.2::1q25>1qter)[2]/46,XY,t(3;9)(3pter>3q21::9q13>9qter;9pter>9q13::3q21>3qter)[22]/46,XY[23]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
13 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY,t(5;12)(5qter>5p15.3::12q13>12qter;12pter>12q13::5p15.3>5pter)[1]/45,X[2]/46,XY,t(1;5;18)(1pter>1q25::5q11.2>5qter;5pter>5q11.2::18q11.2>18qter;18pter>18q11.2::1q25>1qter)[2]/46,XY,t(3;9)(3pter>3q21::9q13>9qter;9pter>9q13::3q21>3qter)[22]/46,XY[23]. Donor is the cousin of AG04456. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Schneider EL, Monticone R, Smith J, Braunschweiger K, Roberts T, Skin fibroblast cultures derived from members of the Baltimore longitudinal study: a new resource for studies of cellular aging. Cytogenet Cell Genet31:40-6 1981 |
| PubMed ID: 7307581 |
| Cumulative PDL at Freeze |
13 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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