AG04393
Fibroblast from Lung, Lung
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
12 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The culture was initiated on 6/25/79 from explants of minced lung tissue taken from a therapeutically aborted fetus of 16 weeks gestational age. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. A matched skin-derived fibroblast culture from same fetus is AG04392. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Chang HY, Chi JT, Dudoit S, Bondre C, van de Rijn M, Botstein D, Brown PO, Diversity, topographic differentiation, and positional memory in human fibroblasts. Proc Natl Acad Sci U S A99(20):12877-82 2002 |
| PubMed ID: 12297622 |
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| Jacobs L, Bean CL, Marx JA, Optimal phenotypic expression times for HPRT mutants induced in foreskin-, skin-, and lung-derived human diploid fibroblasts. Environ Mutagen5:717-31 1983 |
| PubMed ID: 6617601 |
| Cumulative PDL at Freeze |
12 |
| Passage Frozen |
3 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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