NG12207
DNA from Fibroblast
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
| Quantity |
10 µg |
| Quantitation Method |
Please see our FAQ |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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DNA from Fibroblast
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
4.99 |
| Passage Frozen |
12 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The donor was diagnosed with a benign lesion (hamartoma). The culture was established on 1/10/80 using explants of minced skin tissue taken from the base of the forearm. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY/46,XY,der(1)t(1;11)(q44;q13)/46,XY,del(6) (p11.2)/46,XY,dup(9q); 84%/8%/4%/4% with 6% of the cells examined showing random chromosome loss/gain and 10% showing random chromosomal aberrations. Culture was frozen at passage 9 and the PDL is unknown. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
| PubMed ID: 30567591 |
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