Description:
CHROMOSOME 13 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
Repository
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No Data
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Subcollection |
Hybrids |
Sample Source
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No Data
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Subject Type
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No Data
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Family Type
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No Data
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Ethnicity
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No Data
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Family Member
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No Data
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Genetic Data
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No Data
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 13q14.3, RB1, 13q11-q12, D13S232, and 13q34, F7.1, and gave a negative result with a primer for 13q22-q31, D13S317. |
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Warburton D, Yu MT, Tantravahi U, Lee C, Cayanis E, Russo J, Fischer SG, Regional localization of 32 NotI-HindIII fragments from a human chromosome 13 library by a somatic cell hybrid panel and in situ hybridization. Genomics16:355-60 1993 |
PubMed ID: 8314575 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
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