Description:
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
Repository
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No Data
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Subcollection |
Hybrids |
Sample Source
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No Data
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Subject Type
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No Data
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Family Type
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No Data
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Ethnicity
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No Data
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Family Member
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No Data
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Genetic Data
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No Data
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.3, DXS1003, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD, and gave a negative result with a primer for Xp22.32, STS. |
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GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, 2q13, IL1A, and 2q37, ALPP. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 4p15.3, QDPR, and 4q31-q33, 4S1626. |
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Harun RB, Smith KK, Leek JP, Markham AF, Norris A, Morrison JF, Characterization of human SHC p66 cDNA and its processed pseudogene mapping to Xq12-q13.1. Genomics42:349-52 1997 |
PubMed ID: 9192859 |
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Mills KA, Buetow KH, Xu Y, Ritty TM, Mathews KD, Bodrug SE, Wijmenga C, Balazs I, Murray JC, Genetic and physical mapping on chromosome 4 narrows the localization of the gene for facioscapulohumeral muscular dystrophy (FSHD). Am J Hum Genet51:432-9 1992 |
PubMed ID: 1642243 |
Split Ratio |
1:4 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Aminopterin 4 x 10-7M |
Supplement |
Hypoxanthine 1 x 10-4M |
Supplement |
Thymidine 1.6 x 10-5M |
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