NA10659
DNA from Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 15 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Sample Source
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DNA from Somatic cell hybrid
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Mouse/human hybrid retaining human chromosomes #3, 6, 7, 11, 12, 14, 18, 19, 20, 22, & der(17)t(15;17)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 15q21-q22.2, B2M, 15q11-q12, D15S11, and 15q11.2-q12, GABRB3, and gave a positive result with a primer for 15q23, D15S1485A. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2 and gave a negative result with a probe for 17q25, P4HB. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 17p13.1, TP53, and gave negative results with primers for 17q21.3-q23, MPO1, 17q, GAS, and 17q25-qter, D17S784. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 19p13.1, D19S11, and 19q13.3, LHB. |
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| Remarks |
Line P12.3B5; produced by fusion of bone marrow cells from an APL pt, t(15;17) (q22;q11.2or12), with TK-def Cl1D mouse cells; selected in HAT medium with ouabain; grown without selection; 88% retain the der(17) & other human chromos |
| Moran JL, Johnston SH, Rauskolb C, Bhalerao J, Bowcock AM, Vogt TF, Genomic structure, mapping, and expression analysis of the mammalian Lunatic,
Manic, and Radical fringe genes. Mamm Genome10(6):535-41 1999 |
| PubMed ID: 10341080 |
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| Pelletier GJ, Brody SL, Liapis H, White RA, Hackett BP, A human forkhead/winged-helix transcription factor expressed in developing pulmonary and renal epithelium. Am J Physiol274:L351-9 1998 |
| PubMed ID: 9530170 |
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| Verdier AS, Mattei MG, Lovec H, Hartung H, Goldfarb M, Birnbaum D, Coulier F, Chromosomal mapping of two novel human FGF genes, FGF11 and FGF12. Genomics40:151-4 1997 |
| PubMed ID: 9070933 |
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| Brody LC, Abel KJ, Castilla LH, Couch FJ, McKinley DR, Yin G, Ho PP, Merajver S, Chandrasekharappa SC, Xu J, et al, Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17. Genomics25:238-47 1995 |
| PubMed ID: 7774924 |
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| Carr IM, Markham AF, Molecular genetic analysis of the human sorbitol dehydrogenase gene Mammalian genome : official journal of the International Mammalian Genome Society6:645-52 1995 |
| PubMed ID: 8535074 |
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| Flejter WL, Bennett-Baker P, Barcroft CL, Kiousis S, Chamberlain JS, Region-specific cosmids and STRPs identified by chromosome microdissection and FISH. Genomics25:413-20 1995 |
| PubMed ID: 7789975 |
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| Hobson GM, Krahe R, Garcia E, Siciliano MJ, Funanage VL, Regional chromosomal assignments for four members of the MADS domain transcription enhancer factor 2 (MEF2) gene family to human chromosomes 15q26, 19p12, 5q14, and 1q12-q23. Genomics29:704-11 1995 |
| PubMed ID: 8575763 |
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| Polymeropoulos MH, Torres R, Yanovski JA, Chandrasekharappa SC, Ledbetter DH, The human corticotropin-releasing factor receptor (CRHR) gene maps to chromosome 17q12-q22. Genomics28:123-4 1995 |
| PubMed ID: 7590738 |
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| Chang DY, Nelson B, Bilyeu T, Hsu K, Darlington GJ, Maraia RJ, A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA. Mol Cell Biol14:3949-59 1994 |
| PubMed ID: 8196634 |
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| Driscoll CT, Darlington GJ, Maraia RJ, The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog
exclusion probing of somatic cell hybrid RNA. Nucleic Acids Res22(5):722-5 1994 |
| PubMed ID: 8139910 |
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| O'Connell P, Albertsen H, Matsunami N, Taylor T, Hundley JE, Johnson-Pais TL, Reus B, Lawrence E, Ballard L, White R, et al, A radiation hybrid map of the BRCA1 region. Am J Hum Genet54:526-34 1994 |
| PubMed ID: 8116622 |
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| Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, Green ED, Kelly TJ, High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3). Genomics20:249-57 1994 |
| PubMed ID: 8020972 |
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| Anderson LA, Friedman L, Osborne-Lawrence S, Lynch E, Weissenbach J, Bowcock A, King MC, High-density genetic map of the BRCA1 region of chromosome 17q12-q21. Genomics17:618-23 1993 |
| PubMed ID: 8244378 |
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| van Tuinen P, Rich DC, Summers KM, Ledbetter DH, Regional mapping panel for human chromosome 17: application to neurofibromatosis type 1. Genomics1:374-81 1987 |
| PubMed ID: 3130306 |
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