Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
Apparently healthy iPSCs
PIGI Consented Sample
Protocols Protocol PDF
Cell Type Stem cell
Tissue Type Induced pluripotent stem cell
Transformant Reprogrammed (Episomal)
Race Caucasian
Family Member 1
Family History N
Relation to Proband proband
ISCN 46,XY[24].arr(1-22)x2,(X,Y)x1
Species Homo sapiens
Common Name Human
Remarks Participant (huAA53E0) in the Personal Genome Project: http://www.personalgenomes.org history of Blue rubber bleb nevus syndrome; central serous chorioretinopathy; cystoid macular degeneration; hemangioma; migraine with aura; narcolepsy; sleep paralysis; same subject as GM24385 (lymph); mother is GM24143 (Lymph) / GM26077 (stem cell); father is GM24149 (Lymph); Note: Detection of exogenous LIN28 expression.
Passage Frozen 14
 
Induced Pluripotent Stem Cell The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.
 
Remark Participant (huAA53E0) in the Personal Genome Project: http://www.personalgenomes.org history of Blue rubber bleb nevus syndrome; central serous chorioretinopathy; cystoid macular degeneration; hemangioma; migraine with aura; narcolepsy; sleep paralysis; same subject as GM24385 (lymph); mother is GM24143 (Lymph) / GM26077 (stem cell); father is GM24149 (Lymph); Note: Detection of exogenous LIN28 expression.
Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016
PubMed ID: 27724973
No data is available
Passage Frozen 14
Split Ratio 1:6
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent
Serum 20% Knock-out Serum Replacement Not inactivated
Substrate Gelatin + Feeder Layer
Supplement Basic Fibroblast Growth Factor 10ng/ml