Passage Frozen	14
Induced Pluripotent Stem Cell	The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

Remark

Participant (huAA53E0) in the Personal Genome Project: http://www.personalgenomes.org history of Blue rubber bleb nevus syndrome; central serous chorioretinopathy; cystoid macular degeneration; hemangioma; migraine with aura; narcolepsy; sleep paralysis; same subject as GM24385 (lymph); mother is GM24143 (Lymph) / GM26077 (stem cell); father is GM24149 (Lymph); Note: Detection of exogenous LIN28 expression.

Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016

PubMed ID: 27724973

No data is available

No data is available

Passage Frozen	14
Split Ratio	1:6
Temperature	37 C
Percent CO2	5%
Percent O2	AMBIENT
Medium	Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent
Serum	20% Knock-out Serum Replacement Not inactivated
Substrate	Gelatin + Feeder Layer
Supplement	Basic Fibroblast Growth Factor 10ng/ml