Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently healthy iPSCs
Apparently Healthy Collection
Protocols Protocol PDF
Biopsy Source Peripheral vein
Cell Type Stem cell
Tissue Type Induced pluripotent stem cell
Transformant Reprogrammed (Episomal)
Race Caucasian
Family Member 1
Relation to Proband proband
Confirmation Karyotypic analysis and Case history
ISCN 46,XX
Species Homo sapiens
Common Name Human
Remarks Induced pluripotent stem cell line derived from GM02254 Lymphoid
Induced Pluripotent Stem Cell The cell line submitted to the Repository frozen was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and the PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSCs and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.
 
Remark Induced pluripotent stem cell line derived from GM02254 Lymphoid
No data is available
No data is available
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium mTeSR1
Serum none
Substrate Matrigel