GM20366
LCL from B-Lymphocyte
Description:
ISODICENTRIC CHROMOSOME
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Chromosome Abnormalities dbGaP |
Biopsy Source
|
Peripheral vein
|
Cell Type
|
B-Lymphocyte
|
Tissue Type
|
Blood
|
Transformant
|
Epstein-Barr Virus
|
Sample Source
|
LCL from B-Lymphocyte
|
Race
|
White
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis before cell line submission to CCR
|
ISCN
|
47,XY,+idic(15)(q13).ish idic(15)(q13)(D15Z1++,D15S11++,GABRB3++).arr 15q11.2q13.3(20224750-30408507)x4
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
|
Cytogenetics |
Chromosome 15: DICENTRIC CHROMOSOME Aneuploid Segment (+)15pter>15q13 |
Remarks |
Testing (Bayley) showed mental age of 11 months; autism on ADI-R; seizures |
Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013 |
PubMed ID: 23665875 |
View |
FISH GM20366:
D15S11 probe:
Spectrum Green detects 15p11.2;
Spectrum Orange detects q11-13;
Dapi counterstain |
|
FISH GM20366:
D15S11 probe:
Spectrum Green detects 15p11.2;
Spectrum Orange detects q11-13;
Dapi counterstain |
|
FISH GM20366:
GABRB3 probe:
Spectrum Green detects 15p11.2;
Spectrum Orange detects q11-13;
Dapi counterstain |
|
FISH GM20366:
GABRB3 probe:
Spectrum Green detects 15p11.2;
Spectrum Orange detects q11-13;
Dapi counterstain |
|
karyotype GM20366:
GABRB3 probe:
Spectrum Green detects 15p11.2;
Spectrum Orange detects q11-13;
Dapi counterstain |
|
karyotype GM20366:
GABRB3 probe:
Spectrum Green detects 15p11.2;
Spectrum Orange detects q11-13;
Dapi counterstain |
|
karyotype GM20366:
GABRB3 probe:
Spectrum Green detects 15p11.2;
Spectrum Orange detects q11-13;
Dapi counterstain |
|
karyotype GM20366:
GABRB3 probe:
Spectrum Green detects 15p11.2;
Spectrum Orange detects q11-13;
Dapi counterstain |
|
karyotype GM20366:
GABRB3 probe:
Spectrum Green detects 15p11.2;
Spectrum Orange detects q11-13;
Dapi counterstain |
Split Ratio |
1:2 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not Inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
Supplement |
- |
|
|