GM14562
Somatic cell hybrid
Description:
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining a human del(18)(q22.1) in 100% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 18p11.1-p11.2, D18S44, 18p11.31-p11.22, TYMS, and 18q12.1, TTR, and gave a negative result with a primer for 18q23, D18S1390. |
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| Remarks |
Line JH282; produced by the fusion of human lymphoblasts, GM50120 (JL181) with karyotype del(18)(pter> q22.1:), with Chinese hamster UCW206 cells; grown at 39C; 100% of cells retain a human del(18)(q22.1) |
| Rojas K, Overhauser J, Sublocalization of 21 chromosome 18-specific microsatellite markers. Genomics18:169-71 1993 |
| PubMed ID: 8276412 |
| Split Ratio |
1:25 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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