GM13081
Somatic cell hybrid
Description:
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/Chinese hamster hybrid retaining a human der(3)t(3;18)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 3p25, RAF1. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 3q26.2-q27, GLUT2, and gave a positive result with a primer for 3p22-p21.33, |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 18q23, MBP, and gave a negative result with a probe for 18q12.3, D18S6. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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| Remarks |
Line JH281; produced by the fusion of human cells, 46,XX,t(3;18)(q27;q21.1), with Chinese hamster UCW206 cells; grown in medium with 0.2mM proline at 39C; retains human der(3)t(3;18) in 96% of cells |
| Lin Y, Sheridan PL, Jones KA, Evans GA, The HIP116 SNF2/SW12-related transcription factor gene (SNF2L3) is located on human chromosome 3q25.1-q26.1. Genomics27:381-2 1995 |
| PubMed ID: 7558014 |
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| van Kessel AG, Straub RE, Silverman GA, Gerken S, Overhauser J, Report and abstracts of the second international workshop on human chromosome 18 mapping. Doorwerth, the Netherlands, July 19-20, 1993. Cytogenet Cell Genet65:142-65 1994 |
| PubMed ID: 8222751 |
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| Rojas K, Straub RE, Kurtz A, Feder M, Mewar R, Gilliam TC, Overhauser J, Identification and localization of microsatellite markers covering human chromosome 18. Genomics14:1095-7 1992 |
| PubMed ID: 1478651 |
| Split Ratio |
1:25 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Percent O2 |
AMBIENT |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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