GM11980
Somatic cell hybrid
Description:
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Chinese hamster/human hybrid retaining human der(1)t(1;21)(p31;q22), 19, & Y
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 1p36, PND, and gave a positive result with a probe for 1q32, REN. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 1p13, NGFB, 1q32, REN, and 1q44, SV-1_2004, and gave a negative result with a primer for 1p36.2, TNFR2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5pterp15.3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 11q23.3, ETS1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 13q34, D13S3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 21q22.1q22.2, D21S17. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 21q21.2, APP, and 21q22.3, ITGB2, and gave negative results with primers for 21q11, D21S120, and 21q11.1, D21S236. |
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| Remarks |
Line 1881C-13b; human fibro, GM01881, 46,XY,t(1;21)(p31;q22), fused with Chinese hamster ovary K1 mutant Ade C cells; grown in hypoxanthine-free medium with 7% dialyzed FBS; retains der(1) in 100% of cells & other human chromosomes |
| Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
| PubMed ID: 12829847 |
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| Yu J, Hartz J, Xu Y, Gemmill RM, Korenberg JR, Patterson D, Kao FT, Isolation, characterization, and regional mapping of microclones from a human chromosome 21 microdissection library. Am J Hum Genet51:263-72 1992 |
| PubMed ID: 1642227 |
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| Gardiner K, Watkins P, Munke M, Drabkin H, Jones C, Patterson D, Partial physical map of human chromosome 21. Somat Cell Mol Genet14:623-37 1988 |
| PubMed ID: 3194801 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
7% dialyzed fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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