GM11936
Somatic cell hybrid
Description:
CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Mouse/human hybrid retaining human der(16)t(11;16)(q13;p11)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 4p16.2p15.1, D4S20. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 11p15.5, D11S12, and gave a positive result with a probe for 11q23.3, ETS1. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 11p13, CAT, and gave a positive result with a primer for 11q21-q22, CLG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 12q14q24.1, D12S7. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
Dna from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 14q32.1q32.3, D14S13. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 16q13q22.1, MT2, and gave a negative result with a probe for 16pterp13, D16S36. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 16q22.1, LCAT, 16q23.2, D16S266, 16q24.3, PRSM1, and gave a negative result with a primer for 16p13.3, HBA. |
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| Remarks |
Line CF52-46-6; produced by the fusion of human fibroblast, GM03005, 46,XX,t(11;16) (q13;p11), with HPRT-deficient mouse A9 cells; grown in medium with adenine, aminopterin, and thymidine; retains human der(16)t(11;16) in 100% of cells |
| O'Keefe DS, Bacich DJ, Heston WD, Comparative analysis of prostate-specific membrane antigen (PSMA) versus a prostate-specific membrane antigen-like gene The Prostate58:200-10 2004 |
| PubMed ID: 14716746 |
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| O'Keefe DS, Su SL, Bacich DJ, Horiguchi Y, Luo Y, Powell CT, Zandvliet D, Russell PJ, Molloy PL, Nowak NJ, Shows TB, Mullins C, Vonder Haar RA, Fair WR, Heston WDW, Mapping, genomic organization and promoter analysis of the human prostate-specific membrane antigen gene [In Process Citation] Biochim Biophys Acta1443:113-27 1998 |
| PubMed ID: 9838072 |
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| Leek J, Lench N, Maraj B, Bailey A, Carr IM, Andersen S, Cross J, Whelan P, MacLennan KA, Meredith DM, Prostate-specific membrane antigen: evidence for the existence of a second related human gene British journal of cancer72:583-8 1995 |
| PubMed ID: 7669565 |
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| Junien C, van Heyningen V, Evans G, Little P, Mannens M, Report of the second chromosome 11 workshop. Genomics12:620-5 1992 |
| PubMed ID: 1348491 |
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| Julier C, Nakamura Y, Lathrop M, O'Connell P, Leppert M, Litt M, Mohandas T, Lalouel JM, White R, A detailed genetic map of the long arm of chromosome 11. Genomics7:335-45 1990 |
| PubMed ID: 1973139 |
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| Koeffler HP, Sparkes RS, Stang H, Mohandas T, Regional assignment of genes for human alpha-globin and phosphoglycollate phosphatase to the short arm of chromosome 16. Proc Natl Acad Sci U S A78:7015-8 1981 |
| PubMed ID: 6273902 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Adenine 7.5 x 10-5M |
| Supplement |
Aminopterin 1.6 x 10-6M |
| Supplement |
Thymidine 3.2 x 10-5M |
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