GM11750
Somatic cell hybrid
Description:
CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Mouse/human hybrid retaining human der(X)t(X;3)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.3, DXS1003, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD, and gave a negative result with a primer for Xp22.32, STS. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 1p13, NGFB. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, and 3p25-p24.2, D3S1304, and gave negative results with primers for 3q13.1-q13.2, D3S1310, 3q22-q24, D3S1764, 3q26.2-q27, GLUT2, and 3q28q29, D3S1311. |
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| Remarks |
Line R1-1; produced by the fusion of human lymphoblasts, der(X)t(X;3)(3pter> 3p13::Xp2?>Xqter), with mouse RAG cells; grown in HAT medium; retains human 3pter>p13 in 92% of cells |
| Maassen VanDenBrink A, Vergouwe MN, Ophoff RA, Naylor SL, Dauwerse HG,
Saxena PR, Ferrari MD, Frants RR, Chromosomal localization of the 5-HT1F receptor gene: no evidence for
involvement in response to sumatriptan in migraine patients. Am J Med Genet77(5):415-20 1998 |
| PubMed ID: 9632173 |
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| Ayyanathan K, Naylor SL, Kunapuli SP, Structural characterization and fine chromosomal mapping of the human P2Y1 purinergic receptor gene (P2RY1). Somat Cell Mol Genet22:419-24 1996 |
| PubMed ID: 9039850 |
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| Bailey A, Norris AL, Leek JP, Clissold PM, Carr IM, Ogilvie DJ, Morrison JF, Meredith DM, Markham AF, Yeast artificial chromosome cloning of the beta-catenin locus on human chromosome 3p21-22. Chromosome Res3:201-3 1995 |
| PubMed ID: 7780664 |
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| Naylor SL, Buys CH, Carritt B, Report and abstracts of the Fourth International Workshop on Human Chromosome 3 Mapping Cytogenetics and cell genetics65:2-50 1994 |
| PubMed ID: 8104766 |
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| Drabkin H, Wright M, Jonsen M, Varkony T, Jones C, Sage M, Gold S, Morse H, Mendez M, Erickson P, Development of a somatic cell hybrid mapping panel and molecular probes for human chromosome 3. Genomics8:435-46 1990 |
| PubMed ID: 2286371 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
7% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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