GM11692
Somatic cell hybrid
Description:
CHROMOSOME 8 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
Human/Chinese hamster somatic cell hybrid retaining a human 8pter>8q11 attached to rodent seg & other hum chromos
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 8q22q23, D8S5, and 8q22, CA2, and gave a positive result with a probe for 8p21, NEFL. M. Wagner, Research Triangle Park, NC, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p22, D8S258, and 8p21.3-cen, D8S259, and gave negative results with primers for 8q11, MOS, 8q13q22.1, D8S286, 8q22.2, D8S271, 8q22.3, D8S276, and 8q24.3-qter, D8S263. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p12, D8S87, 8p23-p22, DEF1, 8p21-p11.2, LHRH, and 8q11, MOS, and gave negative results with primers for 8q11.22-q12, D8S260, 8q22.2, D8S270, 8q23-q24, D8S199, and 8q24, TG. |
| |
| Remarks |
Subclone 14 of line 1SHL3; produced by the fusion of normal human lymphocytes with Chinese hamster ovary irs-1 cells; grown in medium without selection; retains human 8pter>8q11 attached to a rodent chromo segment & other hum chromos |
| Wagner MJ, Ge Y, Siciliano M, Wells DE, A hybrid cell mapping panel for regional localization of probes to human chromosome 8. Genomics10:114-25 1991 |
| PubMed ID: 2045096 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|