GM11223
Somatic cell hybrid
Description:
CHROMOSOME 22 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 1 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis and In situ hybridization
|
|
ISCN
|
Human/Chinese hamster hybrid retaining hum der(22)t(1;22) (92% of cells) & #3, 4, 8, 11, 12, 14, 17, 18, 19, 20, & 21
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 1q32, REN, and 1p36, PND. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 1p13, NGFB, and 1q32, REN. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 11p13, CAT, and 11q23.3, ETS1. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 20q13.2, D20S4. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 22q11.2, IGLC2, and 22q12.1-q13.1, D22S300. |
| |
| Remarks |
Line 1/22 AM-27; produced by the fusion of human leukocytes, t(1;22)(q42;q13.1), with Chinese hamster a3 cells; grown in HAT medium; retains human der(22)t(1;22) in 92% of cells & other human chromosomes |
| Matthijs G, Schollen E, Pirard M, Budarf ML, Van Schaftingen E, Cassiman JJ, PMM (PMM1), the human homologue of SEC53 or yeast phosphomannomutase, is localized on chromosome 22q13. Genomics40:41-7 1997 |
| PubMed ID: 9070917 |
| |
| Budarf ML, Eckman B, Michaud D, McDonald T, Gavigan S, Buetow KH, Tatsumura Y, Liu Z, Hilliard C, Driscoll D, Goldmuntz E, Meese E, Zwarthoff EC, Williams S, McDermid H, Dumanski JP, Biegel J, Bell CJ, Emanuel BS, Regional localization of over 300 loci on human chromosome 22 using a somatic cell hybrid mapping panel. Genomics35:275-88 1996 |
| PubMed ID: 8661140 |
| |
| Bell CJ, Budarf ML, Nieuwenhuijsen BW, Barnoski BL, Buetow KH, Campbell K, Colbert AM, Collins J, Daly M, Desjardins PR, et al, Integration of physical, breakpoint and genetic maps of chromosome 22. Localization of 587 yeast artificial chromosomes with 238 mapped markers. Hum Mol Genet4:59-69 1995 |
| PubMed ID: 7711735 |
| |
| Budarf ML, Perier F, Barnoski BL, Bell CJ, Vandenberg CA, Assignment of the human hippocampal inward rectifier potassium channel (HIR) gene to 22q13.1. Genomics26:625-9 1995 |
| PubMed ID: 7607694 |
| |
| Bucan M, Gatalica B, Nolan P, Chung A, Leroux A, Grossman MH, Nadeau JH, Emanuel BS, Budarf M, Comparative mapping of 9 human chromosome 22q loci in the laboratory mouse. Hum Mol Genet2:1245-52 1993 |
| PubMed ID: 8401507 |
| |
| Morris C, Courtay C, Geurts van Kessel A, ten Hoeve J, Heisterkamp N, Groffen J, Localization of a gamma-glutamyl-transferase-related gene family on chromosome 22. Hum Genet91:31-6 1993 |
| PubMed ID: 8095916 |
| |
| Adham IM, Grzeschik KH, Geurts van Kessel AH, Engel W, The gene encoding the human preproacrosin (ACR) maps to the q13-qter region on chromosome 22. Hum Genet84:59-62 1989 |
| PubMed ID: 2606479 |
| |
| Geurts van Kessel AH, Westerveld A, de Groot PG, Meera Khan P, Hagemeijer A, Regional localization of the genes coding for human ACO2, ARSA, and NAGA on chromosome 22. Cytogenet Cell Genet28:169-72 1980 |
| PubMed ID: 7192199 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
|
|