AG20623

Overview

Repository

NIA Aging Cell Culture Repository

Subcollection

Apparently Healthy Collection

Biopsy Source

Peripheral vein

Cell Type

B-Lymphocyte

Tissue Type

Blood

Transformant

Epstein-Barr Virus

Sample Source

LCL from B-Lymphocyte

Race

White

Relation to Proband

proband

Confirmation

Clinical summary/Case history

ISCN

47,XX,+12[25]/46,XX,t(2;7)(2qter>2p13::7q22>7qter;7pter>7q22::2p13>2pter)[4]/46,XX[21]

Species

Homo sapiens

Common Name

Human

Remarks

Donor reports anemia, gall stones, hysterectomy, slight hearing loss with aids in both ears, cataracts removed by surgery, hand tremor, and memory loss (atributed by husband to diagnosed early stage Alzheimer disease); family history of hypertension (mother); never smoked; a sister is 90 years of age

Characterizations

IDENTIFICATION OF SPECIES OF ORIGIN

Species of Origin confirmed by LINE assay

Publications

Bracci AN, Dallmann A, Ding Q, Hubisz MJ, Caballero M, Koren A, The evolution of the human DNA replication timing program Proceedings of the National Academy of Sciences of the United States of America120:e2213896120 2023

PubMed ID: 36848554

Caballero M, Ge T, Rebelo AR, Seo S, Kim S, Brooks K, Zuccaro M, Kanagaraj R, Vershkov D, Kim D, Smogorzewska A, Smolka M, Benvenisty N, West SC, Egli D, Mace EM, Koren A, Comprehensive analysis of DNA replication timing across 184 cell lines suggests a role for MCM10 in replication timing regulation Human molecular genetics120:e2213896120 2021

PubMed ID: 35394024

Culture Protocols

Split Ratio

1:3

Temperature

37 C

Percent CO2

Medium

Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent

Serum

15% fetal bovine serum Not Inactivated

Substrate

None specified

Subcultivation Method

dilution - add fresh medium

Supplement

Description:

NIA AGING CELL REPOSITORY'S LONGEVITY RESEARCH PROGRAM

Affected:

No

Sex:

Female

Age:

92 YR (At Sampling)

Overview

Characterizations

Phenotypic Data

Publications

Culture Protocols