AG08351
Smooth muscle from Aorta, thoracic
Description:
MACACA
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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Permit Requirements
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CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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42,XY
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Species
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Macaca nemestrina
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Common Name
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pigtailed macaque
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Remarks
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| PDL at Senescence |
54 |
| PDL at Freeze |
4 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remark |
The thoracic aorta was removed post-mortem on 7/25/85 from a fetus of 130 days gestational age. Strips of the intimal and medial layers of the vessel were removed, minced, and used as explants to initiate the culture. Cell morphology is fibroblast-like. The karyotype is 42,XY; normal diploid male. Other cultures from this monkey are AG08346 and AG08347. |
| PDL at Senescence |
54 |
| Cumulative PDL at Freeze |
4 |
| Passage Frozen |
2 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
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