GM16260
Tumor-derived cell line from Bladder carcinoma
Description:
ANEUPLOID CHROMOSOME NUMBER - NON-TRISOMIC
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities
Engineered Cell Cultures |
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Biopsy Source
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Bladder carcinoma
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Cell Type
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Tumor-derived cell line
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Tissue Type
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Tumor
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Transformant
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Untransformed
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Sample Source
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Tumor-derived cell line from Bladder carcinoma
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,X,del(4)(pter>q31::q33>qter),der(8)(:7q35>7q32::8p12>8qter),del(9)(:p21>qter),der(10)(10qter>10p11::22q12>22qter),t(15;18)(15pter>15q21::18p11>18pter;18qter>18p11::15q21>15qter),der(20)(20qter>20p11::11p11>11pter),del(22)(pter>q12:).rev ish dim(4)(q31>q33),enh(7)(q33>q35),dim(8)(p12>pter),dim(9)(p21>pter),dim(10)(p11>pter),enh(11)(p11>pter),enh(20)(p11>qter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
10 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
A spontaneous HPRT- mutant of a bladder carcinoma line with a quasi-diploid karyotype; GM16260 is monosomic for 4q31>4q33, 8pter>8p12, 9pter>9p21 and 10pter>10p11 |
| Treff NR, Zimmerman R, Bechor E, Hsu J, Rana B, Jensen J, Li J, Samoilenko A, Mowrey W, Van Alstine J, Leondires M, Miller K, Paganetti E, Lello L, Avery S, Hsu S, Melchior Tellier LCA, Validation of concurrent preimplantation genetic testing for polygenic and monogenic disorders, structural rearrangements, and whole and segmental chromosome aneuploidy with a single universal platform European journal of medical genetics62:103647 2019 |
| PubMed ID: 31026593 |
| Passage Frozen |
10 |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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