GM05133
Fibroblast from Fetal, Unspecified
Description:
TRANSLOCATED CHROMOSOME
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Chromosome Abnormalities |
Biopsy Source
|
Unspecified
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Fetal
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Fetal, Unspecified
|
Race
|
Black/African American
|
Family Member
|
3
|
Relation to Proband
|
brother
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
ISCN
|
46,XY,der(14)(14qter> 14p11.2::20p11.1>20pter)mat
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
Passage Frozen |
3 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Cytogenetics |
Chromosome 14: DERIVATIVE CHROMOSOME Aneuploid Segment (-)14pter>14p11 |
|
Chromosome 20: DERIVATIVE CHROMOSOME Aneuploid Segment (+)20pter>20p11 |
|
Chromosome 20: DERIVATIVE CHROMOSOME Trisomic Segment 20pter>20p11 |
Passage Frozen |
3 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|