AG08475
Smooth muscle from Aorta, thoracic
Description:
SUS
ORDER: CETARTIODACTYLA
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Aorta, thoracic
|
|
Cell Type
|
Smooth muscle
|
|
Tissue Type
|
Artery
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Smooth muscle from Aorta, thoracic
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
38,XX
|
|
Species
|
Sus scrofa
|
|
Common Name
|
pig
|
|
Remarks
|
|
| PDL at Senescence |
150 |
| PDL at Freeze |
2 |
| Passage Frozen |
1 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Genbank |
COX1 |
| Remarks |
Large white domestic breed. The age of the donor is estimated. The thoracic aorta was removed immediately post-mortem on 10/15/85. Strips of the medial layer were removed, minced, and used as explants to initiate the culture. Cell morphology is fibroblast-like. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). For lifespan determination this cell line was cultured on gelatin-coated flasks and the medium was supplemented with crude ECGF (0.11mg/ml) and heparin (0.1mg/ml). |
| PDL at Senescence |
150 |
| Cumulative PDL at Freeze |
2 |
| Passage Frozen |
1 |
| Split Ratio |
1:1 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|