AG07684
Endothelial from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, thoracic
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Confirmation
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Clinical summary/Case history
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ISCN
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60,XX
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Senescence |
110 |
| PDL at Freeze |
23 |
| Passage Frozen |
8 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
Holstein breed. The thoracic aorta was obtained on 5/17/79 from a 5 month old fetus. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. Plasma-derived serum was used in the primary culture to select against possible smooth muscle cell contaminants. The cell morphology is polygonal. Karyotype is 60,XX; normal diploid female. The culture tested positive for von Willebrand factor and negative for bovine viruses BVD, PI-3 and BHV-1. Culture, line BFAE-45, medium: HF12 15I. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Mak IT, Chmielinska JJ, Nedelec L, Torres A, Weglicki WB, D-propranolol attenuates lysosomal iron accumulation and oxidative injury in endothelial cells The Journal of pharmacology and experimental therapeutics317:522-8 2006 |
| PubMed ID: 16456084 |
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| Mak IT, Weglicki WB, Potent antioxidant properties of 4-hydroxyl-propranolol. J Pharmacol Exp Ther308(1):85-90 2004 |
| PubMed ID: 14569064 |
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| Chen QR, Zhang L, Luther PW, Mixson AJ, Optimal transfection with the HK polymer depends on its degree of branching and the pH of endocytic vesicles. Nucleic Acids Res30(6):1338-45 2002 |
| PubMed ID: 11884631 |
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| Liu XD, Skold M, Umino T, Zhu YK, Romberger DJ, Spurzem JR, Rennard SI, Endothelial cell-mediated type I collagen gel contraction is regulated by hemin. J Lab Clin Med136(2):100-9 2000 |
| PubMed ID: 10945238 |
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| Loeb AL, O'Brien DK, Longnecker DE, Halothane inhibits bradykinin-stimulated prostacyclin production in endothelial cells. Anesthesiology81:931-8 1994 |
| PubMed ID: 7943843 |
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| Loeb AL, Longnecker DE, Williamson JR, Alteration of calcium mobilization in endothelial cells by volatile anesthetics. Biochem Pharmacol45:1137-42 1993 |
| PubMed ID: 8461042 |
| PDL at Senescence |
110 |
| Cumulative PDL at Freeze |
23 |
| Passage Frozen |
8 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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