AG07683
Endothelial from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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60,XY
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Senescence |
75 |
| PDL at Freeze |
53 |
| Passage Frozen |
10 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Holstein breed. This culture is a clonal derivative of AG07680 obtained by plating single cells at CPD 10. The parental line, AG07680, from a 4 to 5 month old fetus, was initiated on 4/25/79 using cells released by collagenase digestion of the lumen of the thoracic aorta. The karyotype is 60,XY; normal diploid male. The cell morphology is polygonal. This culture tested positive for von Willebrand factor and angiotensin-1 converting enzyme. Culture tested negative for bovine viruses BVD, PI-3 and BHV-1 prior to submission to the Repository. Culture, line BFAE-39K, medium: HF12 15I. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Liu XD, Skold M, Umino T, Zhu YK, Romberger DJ, Spurzem JR, Rennard SI, Endothelial cell-mediated type I collagen gel contraction is regulated by hemin. J Lab Clin Med136(2):100-9 2000 |
| PubMed ID: 10945238 |
| PDL at Senescence |
75 |
| Cumulative PDL at Freeze |
53 |
| Passage Frozen |
10 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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