GM06321
Amniotic fluid-derived cell line from Amniotic fluid
Description:
TRANSLOCATED CHROMOSOME
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Chromosome Abnormalities |
Biopsy Source
|
Amniotic fluid
|
Cell Type
|
Amniotic fluid-derived cell line
|
Transformant
|
Untransformed
|
Sample Source
|
Amniotic fluid-derived cell line from Amniotic fluid
|
Race
|
White
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
ISCN
|
46,XY,t(9;22)(9pter>9q13::22q13.3> 22qter;22pter>22q13.3::9q13>9qter)
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
Passage Frozen |
10 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electophoresis and by Chromosome Analysis |
|
Cytogenetics |
Chromosome 22: TRANSLOCATION Breakpoint 22q13 t(9;22)22q13 |
|
Chromosome 9: TRANSLOCATION Breakpoint 9q13 t(9;22)9q13 |
Remarks |
46,XY,t(9;22)(9pter>9q13::22q13.3> 22qter;22pter>22q13.3::9q13>9qter); balanced |
Passage Frozen |
10 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Amniotic Fluid Culture Medium |
Serum |
none Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|