AG10673
LCL from B-Lymphocyte
Description:
AGED SIB PAIRS COLLECTION
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Aged Sib Pairs
Apparently Healthy Collection |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
|
B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
|
|
Race
|
Black/African American
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
46,XX
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
The culture was initiated on 9/28/89 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. The karyotype is 46,XX with 4% of the cells examined showing random chromosomal aberrations. A lymphoblast culture from the brother of this donor is AG10674A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|