Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Buttock
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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ISCN
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46,XX.arr[hg19](1-22,X)x2
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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|
| PDL at Freeze |
8.11 |
| Passage Frozen |
6 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
Line SK118; skin biopsy (buttock); 46,XX |
| Ferreira LGA, Cabral-da-Silva MC, Pachernegg S, van den Bergen JA, Robevska G, Vlahos K, Howden SE, Ng ES, Dias-da-Silva MR, Sinclair AH, Ayers KL, Generation of heterozygous (MCRIi030-A-1) and homozygous (MCRIi030-A-2) NR2F2/COUP-TFII knockout human iPSC lines Stem cell research76:103374 2023 |
| PubMed ID: 38458031 |
| |
| Burden AF, Manley NC, Clark AD, Gartler SM, Laird CD, Hansen RS, Hemimethylation and non-CpG methylation levels in a promoter region of human LINE-1 (L1) repeated elements The Journal of biological chemistry280:14413-9 2005 |
| PubMed ID: 15710616 |
| |
| Shiraha H, Gupta K, Drabik K, Wells A, Aging fibroblasts present reduced epidermal growth factor (EGF) responsiveness due to preferential loss of EGF receptors [In Process Citation] J Biol Chem275:19343-51 2000 |
| PubMed ID: 10764734 |
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| Russell DW, Hirata RK, Human gene targeting by viral vectors [see comments] Nat Genet18:325-30 1998 |
| PubMed ID: 9537413 |
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| Weiss MJ, Ray K, Fallon MD, Whyte MP, Fedde KN, Lafferty MA, Mulivor RA, Harris H, Analysis of liver/bone/kidney alkaline phosphatase mRNA, DNA, and enzymatic activity in cultured skin fibroblasts from 14 unrelated patients with severe hypophosphatasia. Am J Hum Genet44:686-94 1989 |
| PubMed ID: 2705456 |
| |
| Scott GK, Seow HF, Further evidence for a cell surface proteinase essential to the growth of cultured fibroblasts. Exp Cell Res158:41-52 1985 |
| PubMed ID: 3888646 |
| Passage Frozen |
6 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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