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NA14559 DNA from Somatic cell hybrid

Description:

CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Quantity 0.050mg
Quantitation Method Please see our FAQ
Cell Type Somatic cell hybrid
Transformant Untransformed
Sample Source DNA from Somatic cell hybrid
Confirmation Karyotypic analysis after cell line submission to CCR
ISCN Human/Chinese hamster hybrid retaining a human der(18)t(3;18)(q27;q22.3) in 100% of cells and other human chromosomes
Remarks Line JH251; produced by the fusion of human lymphoblasts, t(3;18)(q27;q22.3), with Chinese hamster UCW206 cells; grown at 39C; 100% of cells retain a human der(18)t(3;18)(q27;q22.3) and other human chromosomes including #3

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp22.32, STS, and Xq21.3, DXS3.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3q13.1-q13.2, D3S1310, and 3q26.2-q27, GLUT2, and gave a positive result with a primer for 3q28-q29, D3S1311.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, 5q13.3-q14, HMGCR, and 5q34-q35, GABRA1.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, and 7q36-qter, D7S550, and gave a positive result with a primer for 7pter-p22, D7S472.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 18p11.1-q11.2, D18S44, 18p11.31-p11.22, TYMS, and 18q12.1, TTR, and gave a negative result with a primer for 18q23, D18S1390.
 

Phenotypic Data

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Remarks Line JH251; produced by the fusion of human lymphoblasts, t(3;18)(q27;q22.3), with Chinese hamster UCW206 cells; grown at 39C; 100% of cells retain a human der(18)t(3;18)(q27;q22.3) and other human chromosomes including #3

Publications

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Rojas K, Overhauser J, Sublocalization of 21 chromosome 18-specific microsatellite markers. Genomics18:169-71 1993
PubMed ID: 8276412

External Links

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dbSNP dbSNP ID: 12118
Pricing
International/Commercial/For-profit:
$449.00USD
U.S. Academic/Non-profit/Government:
$263.00USD
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