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NA13339 DNA from Somatic cell hybrid

Description:

CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Quantity 0.050mg
Quantitation Method Please see our FAQ
Cell Type Somatic cell hybrid
Transformant Untransformed
Sample Source DNA from Somatic cell hybrid
Confirmation Clinical summary/Case history
ISCN Mouse/human somatic cell hybrid retaining human chromosomes 4, 15, 20, & der(X)t(X;3)(q28;q21)
Remarks Line G1A-10; produced by the fusion of human fibro, GM00194, 46,X,t(X;3)(Xpter> Xq28::3q21>3qter), with mouse A9 cells; grown in HAT medium; retains human der (X)t(X;3) in 88% of cells & #4, 15, & 20 in 36%, 40%, & 8% of cells respectively

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.21, ALAS2, Xp11.3, DXS1003, Xp22.32, STS, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 1p13, NGFB.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, 3p25p24.2, D3S1304, and 3q13.1-q13.2, D3S1310, and gave positive results with primers for 3q22-q24, D3S1764, 3q26.2-q27, GLUT2, and 3q28-q29, D3S1311.
 

Phenotypic Data

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Remarks Line G1A-10; produced by the fusion of human fibro, GM00194, 46,X,t(X;3)(Xpter> Xq28::3q21>3qter), with mouse A9 cells; grown in HAT medium; retains human der (X)t(X;3) in 88% of cells & #4, 15, & 20 in 36%, 40%, & 8% of cells respectively

Publications

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Leach RJ, Chinn R, Reus BE, Hayes S, Schantz L, Dubois B, Overhauser J, Ballabio A, Drabkin H, Lewis TB, et al, Regional localization of 188 sequence tagged sites on a somatic cell hybrid mapping panel for human chromosome 3. Genomics24:549-56 1994
PubMed ID: 7713507

External Links

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dbSNP dbSNP ID: 11771
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$449.00USD
Academic/Non-profit/Government:
$263.00USD
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