NA11749
DNA from Somatic cell hybrid
Description:
CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Sample Source
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DNA from Somatic cell hybrid
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining a human der(3)t(3;3)(pter>q21::q26>qter)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 3p25, RAF1, and 3q26.2qter, TFRC. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, 3p25p24.2, D3S1304, 3q13.1-q13.2, D3S1310, 3q26.2-q27, GLUT2, and 3q28q29, D3S1311, and gave a negative result with a primer for 3q22-q24, D3S1764. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16pterp13, D16S36, and 16q13q22.1, MT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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| Remarks |
Line H3-4; produced by the fusion of cells from an AML pt, t(3;3)(q21;q26), with CHO Urd-C cells; grown in uridinedef F12 medium with 5% dialyzed FBS; retains human der(3)t(3;3)(pter>q21::q26> qter) in 84% of cells |
| Ayyanathan K, Naylor SL, Kunapuli SP, Structural characterization and fine chromosomal mapping of the human P2Y1 purinergic receptor gene (P2RY1). Somat Cell Mol Genet22:419-24 1996 |
| PubMed ID: 9039850 |
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| Hess JF, Casselman JT, FitzGerald PG, Chromosomal locations of the genes for the beaded filament proteins CP 115 and CP 47. Curr Eye Res14:11-8 1995 |
| PubMed ID: 7720401 |
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| Lin Y, Sheridan PL, Jones KA, Evans GA, The HIP116 SNF2/SW12-related transcription factor gene (SNF2L3) is located on human chromosome 3q25.1-q26.1. Genomics27:381-2 1995 |
| PubMed ID: 7558014 |
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| Naylor SL, Buys CH, Carritt B, Report and abstracts of the Fourth International Workshop on Human Chromosome 3 Mapping Cytogenetics and cell genetics65:2-50 1994 |
| PubMed ID: 8104766 |
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| Drabkin H, Wright M, Jonsen M, Varkony T, Jones C, Sage M, Gold S, Morse H, Mendez M, Erickson P, Development of a somatic cell hybrid mapping panel and molecular probes for human chromosome 3. Genomics8:435-46 1990 |
| PubMed ID: 2286371 |
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| Drabkin H, Sage M, Helms C, Green P, Gemmill R, Smith D, Erickson P, Hart I, Ferguson-Smith A, Ruddle F, et al, Regional and physical mapping studies characterizing the Greig polysyndactyly 3;7 chromosome translocation, t(3;7)(p21.1;p13). Genomics4:518-29 1989 |
| PubMed ID: 2545596 |
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