NA10115

NA10115 DNA from Somatic cell hybrid

Description:

NIGMS MAPPING PANEL #2, VERSION 3, HUMAN/RODENT SOMATIC CELL HYBRIDS
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MAPPING PANEL #2 DNA
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MINI MAPPING PANEL #2 DNA

Affected:

No Data

Sex:

No Data

Age:

No Data

Overview

Repository

NIGMS Human Genetic Cell Repository

Subcollection

Hybrids

Quantity

0.050mg

Quantitation Method

Please see our FAQ

Cell Type

Somatic cell hybrid

Transformant

Untransformed

Sample Source

DNA from Somatic cell hybrid

Confirmation

Clinical summary/Case history

ISCN

Human/Chinese hamster somatic cell hybrid retaining human chromosome #4

Remarks

Line HHW416; produced by fusing Chinese hamster ovary cells (UCW56) with human leukocytes; retains human chromosome #4 in 96% of cells; hybrid is grown in medium containing 0.2mM proline at 34C

Characterizations

IDENTIFICATION OF SPECIES OF ORIGIN

Species of Origin Confirmed by Chromosome Analysis

GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp22.32, STS, Xq13.3, PGK1, and Xq28, G6PD.

GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME

PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 1p13, NGFB, and 1q32, REN.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2

DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, IL1A. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2q37, ALPP.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3

DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 3p25, RAF1, and 3q26.2qter, TFRC. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p22-p21.33, GLB1, and 3q26.2-q27, GLUT2.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4

DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 4p15.3, QDPR, and 4q11-q13, AFP. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 4p15.3, QDPR, 4p16.3, D4S126, 4q11-q13, AFP, 4q28, FGA, and 4q31-q33, D4S1626.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5

DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 5pterp15.3, D5S10, and 5q33q35, PDGFRB. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6q24-q27, MAS1.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7

DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 7pterp15, ASSP11. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 7p12, EGFR, and gave a negative result with a primer for 7q31.3, CFTR.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8

DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 8p21, NEFL. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8p21-p11.2, LHRH, 8p23-p22, DEF1, and 8q24, TG.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9

DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 9q34, D9S7, & 9q34.1, ABL. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, and 9q22.3-q31, ALDOB. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p21, D9S270, 9pter-p22, D9S178, and 9pter-q12, RLN1.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 10p11.2, ITGB1, and 10q24, PLAU.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11

DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 11q23.3, ETS1. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 11p13, CAT, and 11q21-q22, CLG.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12

DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 12q14q24.1, D12S7. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 12p13.3-p13.2, F8VWF, and 12q22-q24.2, PAH. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 12q22-q23, IGF1.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q14.3, RB1.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 14q11.2, NP, and 14q32.1, PI.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q11.2-q12, GABRB3.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16

DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16q13q22.1, MT2, and 16pterp13, D16S36. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, 16q22.1, HPR, and 16q22.1, LCAT.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17

DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q, GAS, and 17q21.3-q23, MPO.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18

DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 18q12.3, D18S6. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 18p11.1-q11.2, D18S44, and 18q12.1, TTR.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19

DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 19p13.3p13.2, C3, and 19p13.1, D19S11. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 19p13.2, LDLR, and 19q13.2, APOC2.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 20pter-p12, PRNP, and 20q12-q13.11, ADA.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 21q22.3, ITGB2.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 22q11.2, IGLC2.

Phenotypic Data

Remarks

Line HHW416; produced by fusing Chinese hamster ovary cells (UCW56) with human leukocytes; retains human chromosome #4 in 96% of cells; hybrid is grown in medium containing 0.2mM proline at 34C

Publications

Cabianca DS, Casa V, Bodega B, Xynos A, Ginelli E, Tanaka Y, Gabellini D, A long ncRNA links copy number variation to a polycomb/trithorax epigenetic switch in FSHD muscular dystrophy Cell149:819-31 2011

PubMed ID: 22541069

Thomas SN, Waters KM, Morgan WF, Yang AJ, Baulch JE, Quantitative proteomic analysis of mitochondrial proteins reveals prosurvival mechanisms in the perpetuation of radiation-induced genomic instability Free radical biology & medicine149:819-31 2011

PubMed ID: 22569412

Nicklas JA, Pseudogenes of the human HPRT1 gene Environmental and molecular mutagenesis47:212-8 2005

PubMed ID: 16355393

Krona C, Ejeskär K, Carén H, Abel F, Sjöberg RM, Martinsson T, A novel 1p362 located gene, APITD1, with tumour-suppressive properties and a putative p53-binding domain, shows low expression in neuroblastoma tumours British journal of cancer91:1119-30 2004

PubMed ID: 15328517

Mallet F, Bouton O, Prudhomme S, Cheynet V, Oriol G, Bonnaud B, Lucotte G, Duret L, Mandrand B, The endogenous retroviral locus ERVWE1 is a bona fide gene involved in hominoid placental physiology Proceedings of the National Academy of Sciences of the United States of America101:1731-6 2004

PubMed ID: 14757826

Myung K, Ghosh G, Fattah FJ, Li G, Kim H, Dutia A, Pak E, Smith S, Hendrickson EA, Regulation of telomere length and suppression of genomic instability in human somatic cells by Ku86. Mol Cell Biol24(11):5050-9 2004

PubMed ID: 15143195

Conte C, Ebeling M, Marcuz A, Nef P, Andres-Barquin PJ, Identification and characterization of human taste receptor genes belonging to the TAS2R family Cytogenetic and genome research98:45-53 2003

PubMed ID: 12584440

Hansford LM, Smith SA, Haber M, Norris MD, Cheung B, Marshall GM, Cloning and characterization of the human neural cell adhesion molecule, CNTN4 (alias BIG-2) Cytogenetic and genome research101:17-23 2003

PubMed ID: 14571131

Janssens B, Mohapatra B, Vatta M, Goossens S, Vanpoucke G, Kools P, Montoye T, van Hengel J, Bowles NE, van Roy F, Towbin JA, Assessment of the CTNNA3 gene encoding human alpha T-catenin regarding its involvement in dilated cardiomyopathy Human genetics112:227-36 2003

PubMed ID: 12596047

Nagar S, Smith LE, Morgan WF, Mechanisms of cell death associated with death-inducing factors from genomically unstable cell lines. Mutagenesis18(6):549-60 2003

PubMed ID: 14614192

Tarafa G, Prat E, Risques RA, González S, Camps J, Grau M, Guinó E, Moreno V, Esteller M, Herman JG, Germà JR, Miró R, Peinado MA, Capellá G, Common genetic evolutionary pathways in familial adenomatous polyposis tumors Cancer research63:5731-7 2003

PubMed ID: 14522893

Li L, Peterson CA, Kanter-Smoler G, Wei YF, Ramagli LS, Sunnerhagen P, Siciliano MJ, Legerski RJ, hRAD17, a structural homolog of the Schizosaccharomyces pombe RAD17 cell cycle checkpoint gene, stimulates p53 accumulation Oncogene18:1689-99 1999

PubMed ID: 10208430

Alkhayat AH, Kraemer SA, Leipprandt JR, Macek M, Kleijer WJ, Friderici KH, Human beta-mannosidase cDNA characterization and first identification of a mutation associated with human beta-mannosidosis. Hum Mol Genet7:75-83 1998

PubMed ID: 9384606

Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC, Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics47:187-99 1998

PubMed ID: 9479491

Day JP, Limoli CL, Morgan WF, Recombination involving interstitial telomere repeat-like sequences promotes chromosomal instability in Chinese hamster cells. Carcinogenesis19:259-65 1998

PubMed ID: 9498274

Inoue H, Iannotti CA, Welling CM, Veile R, Donis-Keller H, Permutt MA, Human cholecystokinin type A receptor gene: cytogenetic localization, physical mapping, and identification of two missense variants in patients with obesity and non-insulin-dependent diabetes mellitus (NIDDM). Genomics42:331-5 1997

PubMed ID: 9192855

Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997

PubMed ID: 9070934

Kogi M, Fukushige S, Lefevre C, Hadano S, Ikeda JE, A novel tandem repeat sequence located on human chromosome 4p: isolation and characterization. Genomics42:278-83 1997

PubMed ID: 9192848

Liang Y, Chen H, Asher JH Jr, Chang CC, Friedman TB, Human inner ear OCP2 cDNA maps to 5q22-5q35.2 with related sequences on chromosomes 4p16.2-4p14, 5p13-5q22, 7pter-q22, 10 and 12p13-12qter. Gene184:163-7 1997

PubMed ID: 9031623

Schwarz H, Arden K, Lotz M, CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies Biochemical and biophysical research communications235:699-703 1997

PubMed ID: 9207223

Finelli P, Antonacci R, Marzella R, Lonoce A, Archidiacono N, Rocchi M, Structural organization of multiple alphoid subsets coexisting on human chromosomes 1, 4, 5, 7, 9, 15, 18, and 19. Genomics38:325-30 1996

PubMed ID: 8975709

Hirai M, Kusuda J, Hashimoto K, Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. Genomics34(2):263-5 1996

PubMed ID: 8661066

Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995

PubMed ID: 7601467

Bermingham JR Jr, Arden KC, Naumova AK, Sapienza C, Viars CS, Fu XD, Khotz J, Manley JL, Rosenfeld MG, Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics29:70-9 1995

PubMed ID: 8530103

Kermouni A, Van Roost E, Arden KC, Vermeesch JR, Weiss S, Godelaine D, Flint J, Lurquin C, Szikora JP, Higgs DR, The IL-9 receptor gene (IL9R): genomic structure, chromosomal localization in the pseudoautosomal region of the long arm of the sex chromosomes, and identification of IL9R pseudogenes at 9qter, 10pter, 16pter, and 18pter Genomics29:371-82 1995

PubMed ID: 8666384

Slaugenhaupt SA, Roca AL, Liebert CB, Altherr MR, Gusella JF, Reppert SM, Mapping of the gene for the Mel1a-melatonin receptor to human chromosome 4 (MTNR1A) and mouse chromosome 8 (Mtnr1a). Genomics27:355-7 1995

PubMed ID: 7558006

Chang DY, Nelson B, Bilyeu T, Hsu K, Darlington GJ, Maraia RJ, A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA. Mol Cell Biol14:3949-59 1994

PubMed ID: 8196634

De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethé B, Lurquin C, Structure, chromosomal localization, and expression of 12 genes of the MAGE family Immunogenetics40:360-9 1994

PubMed ID: 7927540

Driscoll CT, Darlington GJ, Maraia RJ, The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog exclusion probing of somatic cell hybrid RNA. Nucleic Acids Res22(5):722-5 1994

PubMed ID: 8139910

Ferrin LJ, Camerini-Otero RD, Long-range mapping of gaps and telomeres with RecA-assisted restriction endonuclease (RARE) cleavage. Nat Genet6:379-83 1994

PubMed ID: 8054978

Fukushima A, Okubo K, Sugino H, Hori N, Matoba R, Niiyama T, Murakawa K, Yoshii J, Yokoyama M, Matsubara K, Chromosomal assignment of HepG2 3'-directed partial cDNA sequences by Southern blot hybridization using monochromosomal hybrid cell panels. Genomics22:127-36 1994

PubMed ID: 7959758

Ishida Y, Hadano S, Nagayama T, Tomiyasu H, Wakasa K, Ikeda J, Isolation and characterization of 21 novel expressed DNA sequences from the distal region of human chromosome 4p. Genomics22:302-12 1994

PubMed ID: 7545969

Maraia RJ, Sasaki-Tozawa N, Driscoll CT, Green ED, Darlington GJ, The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genes Nucleic acids research22:3045-52 1994

PubMed ID: 7520568

Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994

PubMed ID: 8275700

Chong SS, Kristjansson K, Zoghbi HY, Hughes MR, Molecular cloning of the cDNA encoding a human renal sodium phosphate transport protein and its assignment to chromosome 6p21.3-p23. Genomics18:355-359 1993

PubMed ID: 8288239

Drwinga HL, Toji LH, Kim CH, Greene AE, Mulivor RA, NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics16:311-4 1993

PubMed ID: 8314568

Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993

PubMed ID: 8478002

Lin B, Rommens JM, Graham RK, Kalchman M, MacDonald H, Nasir J, Delaney A, Goldberg YP, Hayden MR, Differential 3' polyadenylation of the Huntington disease gene results in two mRNA species with variable tissue expression. Hum Mol Genet2:1541-5 1993

PubMed ID: 7903579

Maraia RJ, Driscoll CT, Bilyeu T, Hsu K, Darlington GJ, Multiple dispersed loci produce small cytoplasmic Alu RNA. Mol Cell Biol13:4233-41 1993

PubMed ID: 7686619

Marder BA, Morgan WF, Delayed chromosomal instability induced by DNA damage. Mol Cell Biol13:6667-77 1993

PubMed ID: 8413263

Washington SS, Bowcock AM, Gerken S, Matsunami N, Lesh D, Osborne-Lawrence SL, Cowell J, Ledbetter DH, White RL, Chakravarti A, A somatic cell hybrid map of human chromosome 13. Genomics18:486-95 1993

PubMed ID: 8307557

Yamamoto K, Seto M, Akao Y, Iida S, Nakazawa S, Oshimura M, Takahashi T, Ueda R, Gene rearrangement and truncated mRNA in cell lines with 11q23 translocation. Oncogene8:479-85 1993

PubMed ID: 8426751

Hendricks-Taylor LR, Bachinski LL, Siciliano MJ, Fertitta A, Trask B, de Jong PJ, Ledbetter DH, Darlington GJ, The CCAAT/enhancer binding protein (C/EBP alpha) gene (CEBPA) maps to human chromosome 19q13.1 and the related nuclear factor NF-IL6 (C/EBP beta) gene (CEBPB) maps to human chromosome 20q13.1. Genomics14:12-7 1992

PubMed ID: 1427819

Rao PN, Hayworth R, Akots G, Pettenati MJ, Bowden DW, Physical localization of chromosome 20 markers using somatic cell hybrid cell lines and fluorescence in situ hybridization. Genomics14:532-5 1992

PubMed ID: 1427875

Ledbetter SA, Garcia-Heras J, Ledbetter DH, "PCR-karyotype" of human chromosomes in somatic cell hybrids. Genomics8:614-22 1990

PubMed ID: 2276735

Hayden MR, Hewitt J, Wasmuth JJ, Kastelein JJ, Langlois S, Conneally M, Haines J, Smith B, Hilbert C, Allard D, A polymorphic DNA marker that represents a conserved expressed sequence in the region of the Huntington disease gene. Am J Hum Genet42:125-31 1988

PubMed ID: 2892395

Carlock LR, Smith D, Wasmuth JJ, Genetic counterselective procedure to isolate interspecific cell hybrids containing single human chromosomes: construction of cell hybrids and recombinant DNA libraries specific for human chromosomes 3 and 4. Somat Cell Mol Genet12:163-74 1986

PubMed ID: 3457476

Dana S, Wasmuth JJ, Linkage of the leuS, emtB, and chr genes on chromosome 5 in humans and expression of human genes encoding protein synthetic components in human--Chinese hamster hybrids. Somatic Cell Genet8:245-64 1982

PubMed ID: 9732752

Wasmuth JJ, Chu LY, Linkage in cultured Chinese hamster cells of two genes, emtB and leuS, involved in protein synthesis and isolation of cell lines with mutations in three linked genes. J Cell Biol87:697-702 1980

PubMed ID: 7193212