HG06802

HG06802 iPSC from Blood

Description:

AFRICAN AMERICANS LIVING IN ST. LOUIS, MISSOURI

Affected:

No Data

Sex:

Female

Age:

No Data

Overview

Repository

NHGRI Sample Repository for Human Genetic Research

Subcollection

NHGRI Sample Repository for Human Genetic Research

Protocols

Protocol PDF

Cell Type

Stem cell

Cell Subtype

Induced pluripotent stem cell

Transformant

Reprogrammed (Sendai)

Sample Source

iPSC from Blood

Country of Origin

USA

Family History

Relation to Proband

proband

ISCN

46,XX[20]

Species

Homo sapiens

Common Name

Human

Remarks

These cell lines and DNA samples were prepared from peripheral blood samples collected from people living in the St. Louis, MO metropolitan area who self-identified as African American.The applicable LULL to this line is Sendai-Cyto Tune.

Characterizations

Passage Frozen

Induced Pluripotent Stem Cell

The parental cell line was recovered reprogrammed to an induced pluripotent stem cell line and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

Induced Pluripotent Stem Cell

Phenotypic Data

Remarks

Culture Protocols

Passage Frozen

Split Ratio

1:8

Temperature

37 C

Percent CO2

Percent O2

AMBIENT

Medium

mTeSR1

Serum

none Not inactivated

Substrate

Matrigel

Supplement

Pricing

International/Commercial/For-profit:

$1,789.00USD

U.S. Academic/Non-profit/Government:

$1,110.00USD