Description:
PERSONAL GENOME PROJECT
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently healthy iPSCs
PIGI Consented Sample |
| Protocols |
Protocol PDF |
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Biopsy Source
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Blood
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Sendai)
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Sample Source
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iPSC from Blood
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Race
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More than one race
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Ethnicity
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Hispanic/Latino
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Ethnicity
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Am.Indian/AlaskanNative,French,German,British,Czec
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Family Member
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1
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Family History
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N
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Relation to Proband
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proband
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ISCN
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46,XY[20]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
13 |
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| Induced Pluripotent Stem Cell |
The parental cell line was recovered reprogrammed to an induced pluripotent stem cell line and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
| |
| Remarks |
Participant (hu7123C1) in the Personal Genome Project: http://www.personalgenomes.org
APOE-4 heterozygote; onychomycosis of toenail; hypercholesterolemia; hypertriglyceridemia; mild hypertension; bundle branch block; overweight; borderline glucose intolerance; nephrolithiasis; concussion; shingles; colon polyps; migraine without aura; age-related hearing loss; presbyopia; dental cavities; hemorrhoids; kidney stones; benign prostatic hypertrophy; erectile dysfunction; dandruff; bunions; occipital plagiocephaly (left); procedures include open surgical repair of wrist bone fracture, knee arthroscopy; medications include: Terbinafine, Viagra, Simvastatin, Hydrochlorothiazide, Niacin, Aspirin; mother had Alzheimer’s disease diagnosed in her 80’s; daughter is GM24622 (Lymph). Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is Sendai-CytoTune.
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| Passage Frozen |
13 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
none |
| Substrate |
Matrigel |
| Supplement |
- |
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