Description:
PERSONAL GENOME PROJECT
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently healthy iPSCs
PIGI Consented Sample
Whole-Genome Sequence |
| Protocols |
Protocol PDF |
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Biopsy Source
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Blood
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Sendai)
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Sample Source
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iPSC from Blood
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Race
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Black/African American
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Ethnicity
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AFRICAN-AMERICAN
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY[20]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
15 |
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| Induced Pluripotent Stem Cell |
The parental cell line was recovered reprogrammed to an induced pluripotent stem cell line and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
| |
| Demographic Data |
| Relation to Proband |
proband |
| Age at Sampling |
49 YR |
| Sex |
Male |
| Racial Category |
Black/African American |
| |
| Data Elements |
| Clinical Element Type: Church PHGP |
| (Baseline) |
| Vital Signs |
| Allergies |
| Medications, Vitamins, Supplements |
| Medical History |
| Race, Ethnicity, Ancestry |
| Traits |
| Near-sighted |
No Data |
| Far-sighted |
No Data |
| Wear Prescription Glasses |
No Data |
| Remarks |
Participant #10 (hu604D39) in the Personal Genome Project: http://www.personalgenomes.org
Whole genome sequence data is available on the PGP website; DSP c.1dupA heterozygous, GRIA3 c.-2_-1insG homozygous, INF2 c.2310+8delA heterozygous, SPG7 c.596delG heterozygous. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is Sendai-CytoTune. |
| Passage Frozen |
15 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
none Not inactivated |
| Substrate |
Matrigel |
| Supplement |
- |
|