GM24602
LCL from B-Lymphocyte
Description:
PERSONAL GENOME PROJECT
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection
PIGI Consented Sample
Whole-Genome Sequence |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Country of Origin
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USA
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Family History
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N
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Relation to Proband
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proband
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
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| Remarks |
Participant (hu4BF398) in the Personal Genome Project: http://www.personalgenomes.org
Allergic rhinitis; asthma; age-related cataracts; floaters; benign prostatic hypertrophy; bladder stones; canker sores; dandruff; dental cavities; type 2 diabetes; diverticulitis; dust mite allergy; ear wax impaction; ejaculation difficulty; erectile dysfunction; fatigue; fungal toenail infections; gastroesophageal reflux disease; genital warts; male pattern baldness; hay fever; heartburn; hemorrhoids; hypertension; hiatal hernia; high blood pressure; high cholesterol; high triglycerides; hypothyroidism; impacted tooth; insomnia; kidney stones; measles; mumps; myopia; obesity; occupational hearing loss; periodontal disease; precancerous skin lesions; rosacea; sinus headache; speech disorder (stuttering); tinnitus (ringing in ears); skin tags; severe allergies include: black pepper, garlic, and pollen extracts; medications include: Astelin, Benadryl allergy, Claritin, Allopurinol, Ciclopirox, Aspirin, Efudex, Flurazepam, Lisinopril-Hydrochlorothiazide, Loratadine, Metformin, Metronidazole, Pantoprazole, Ranitidine HCl, Simvastatin, and Tetracycline; procedures include: prostate resection (transurethral), shockwave lithotripsy for kidney stones, and tonsillectomy. Whole genome sequence data is available on the PGP website.
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| Smullen M, Olson MN, Reichert JM, Dawes P, Murray LF, Baer CE, Wang Q, Readhead B, Church GM, Lim ET, Chan Y, Reliable multiplex generation of pooled induced pluripotent stem cells Cell reports methods3:100570 2022 |
| PubMed ID: 37751688 |
| |
| Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016 |
| PubMed ID: 27724973 |
| |
| Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:42 2005 |
| PubMed ID: 16729065 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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