GM24169
LCL from B-Lymphocyte
Description:
PERSONAL GENOME PROJECT
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Apparently Healthy Collection
PIGI Consented Sample |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
|
B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
|
|
Race
|
White
|
|
Ethnicity
|
German, Dutch (Netherlands), British, Spanish
|
|
Family History
|
N
|
|
Relation to Proband
|
proband
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
| |
| Remarks |
Participant (hu599905) in the Personal Genome Project: http://www.personalgenomes.org
Actinic Keratosis; anal fissure; anaphylaxis; asthma; astigmatism; benign prostatic hypertrophy; age-related cataract; constipation; diabetes mellitus, type 2; eczema; gastroesophageal reflux disease; Barrett’s esophagus; Hashimoto’s thyroiditis; hearing loss; hemorrhoids; hiatal hernia; hypercholesterolemia; hypertriglyceridemia; hypertension; cardiac arrhythmia; tinnitus; premature ventricular contractions; knee pain; metabolic syndrome; myopia; NASH, obesity; osteopenia; osteoarthritis; osteoporosis; sciatica; shoulder dislocation; squamous cell sarcinoma; non-melonoma skin cancer; urticaria; colon polyps; hypothyroidism; carpal tunnel syndrome; myopia; presbyopia; dry eye syndrome; floaters; deviated septum; allergic rhinitis; impacted tooth; dental cavities; canker sores (oral ulcers); rectal prolapse; non-alcoholic fatty liver disease; dandruff; allergic contact dermatitis; skin tags; acne; dermatographia; pilonidal cyst; rotator cuff tear; Allergies include canine proteins, cat/feline product derivatives, honey bee venom protein, house dust, mixed vespid venom protein, mold extracts, pollen extracts;medications include Actos, Albuterol, Allegra, low dose Aspirin, Bendadryl, Betamethasone Valerate, Efudex, Epi-Pen (in case of bee sting), Finasteride, Flomax, Flovent HFA, Fluocinonide, Fluticasone, Metformin, Micardis, Nexium, Protopic, Singulair, Synthroid, and Zocor.
|
| Smullen M, Olson MN, Reichert JM, Dawes P, Murray LF, Baer CE, Wang Q, Readhead B, Church GM, Lim ET, Chan Y, Reliable multiplex generation of pooled induced pluripotent stem cells Cell reports methods3:100570 2022 |
| PubMed ID: 37751688 |
| |
| Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016 |
| PubMed ID: 27724973 |
| |
| Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:42 2005 |
| PubMed ID: 16729065 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|