GM24142
LCL from B-Lymphocyte
Description:
PERSONAL GENOME PROJECT
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Apparently Healthy Collection PIGI Consented Sample |
Biopsy Source
|
Peripheral vein
|
Cell Type
|
B-Lymphocyte
|
Tissue Type
|
Blood
|
Transformant
|
Epstein-Barr Virus
|
Sample Source
|
LCL from B-Lymphocyte
|
Race
|
White
|
Ethnicity
|
Swiss, Brittish
|
Family History
|
N
|
Relation to Proband
|
proband
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
|
Remarks |
Participant (huBEDA0B) in the Personal Genome Project: http://www.personalgenomes.org
Benign breast lumps with symptom onset at age range 50-59 years; vascular hemangiomas; subdermal hemangiomas; menarche at 11 years of age; Barrett’s esophagus; presbyopia; floaters; osteopenia; colon polyps; breast fibroadenoma; ocular migraine; nasal polyps; low blood pressure; smoker (less than one pack-year). |
Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016 |
PubMed ID: 27724973 |
|
Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:42 2005 |
PubMed ID: 16729065 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not Inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
Supplement |
- |
|
|