GM24135
LCL from B-Lymphocyte
Description:
PERSONAL GENOME PROJECT
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection
PIGI Consented Sample |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Ethnicity
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Polish, Russian
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Country of Origin
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USA
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Family History
|
N
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
| |
| Remarks |
Participant (hu016B28) in the Personal Genome Project: http://www.personalgenomes.org
Congenital cervical spinal stenosis (symptom onset in 30s); acute cholecystitis; alcohol intolerance; chickenpox; diabetes mellitus, type 2; finger fracture; hypercholesterolemia; hypertriglyceridemia; impacted wisdom teeth; impotence; ingrown toenail; nearsightedness; leg fracture; rubella; sleep apnea; trigger finger; impacted tooth; dental cavities; gingivitis; gastroesophageal reflux disease; diverticulosis; gallstones; colon polyps; myopia; presbyopia; hemorrhoids; deviated septum; allergic rhinitis; kidney stones; urinary tract infection;infertility; dandruff; acne; Achilles tendonitis; calcific tendonitis; procedures include foraminotomy, spinal fusion, cervical laminectomy, tonsillectomy, removal of gall bladder, palatopharyngoplasty, open repair of a broken bone in arm below the elbow; medications include Actoplus Met, Amoxicillin, Bupropion SR, Lipitor, Lisinopril, Neo/Poly/HC, Pantoprazole, Papaverine, and Protonix.
|
| Smullen M, Olson MN, Reichert JM, Dawes P, Murray LF, Baer CE, Wang Q, Readhead B, Church GM, Lim ET, Chan Y, Reliable multiplex generation of pooled induced pluripotent stem cells Cell reports methods3:100570 2022 |
| PubMed ID: 37751688 |
| |
| Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016 |
| PubMed ID: 27724973 |
| |
| Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:42 2005 |
| PubMed ID: 16729065 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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